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RNA Extraction, Reverse Transcription, and Quantitative Real-Time PCR In order to examine the qRT-PCR results, RNAiso-mate Tissue Kit was used to isolate total RNA from frozen fruit tissue (Tiangen, Beijing, China)

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BGI (Yantian District, Shenzhen, China), an established contract research organization, isolated DNA from the remaining cecal samples and generated a metagenomic 16s rDNA amplicon library